 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Extender, Cryoprotectant and Freezing Protocols on Post-thaw Sperm Motility, Morphology and Viability of Three-yellow Cocks (Gallus domesticus) Spermatozoa) ผู้เขียน: วีระศักดิ์ ฟุ้งเฟื่อง,  ดร.วรวิทย์ สิริพลวัฒน์, รองศาสตราจารย์ , Suchart Sa-nguanphan, ดร.อนุชัย ภิญโญภูมิมินทร์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractSemen samples collected from 10 three-yellow cocks (Gallus domesticus) were pooled and treated to determine the effect of extender and cryoprotectant (experiment 1) and the effect of freezing protocol and cryoprotectant (experiment 2) on frozen/thawed semen quality. In the first experiment, semen samples were diluted 1:1 (v:v) with two different semen extenders, Beltsville poultry semen extender (BPSE) or modified Tyrode’s medium (TALP) and cryopreserved with either 6% dimethyl acetamide (DMA) or 8% dimethyl sulfoxide (DMSO). The best result (p<0.05) was obtained for TALP extender + 8% DMSO, with values of 57.32% for viability, 56.57% for normal morphology and 48.50% for motility. In experiment 2, two different freezing protocols (one-step and two-step) were evaluated. The results showed that there was no difference in post-thaw motility and viability of the spermatozoa (sperm) when either the one-step or the two-step protocol was applied with 8% DMSO. In contrast, the sperm motility and viability with the one-step protocol were better (p<0.05) than those with the two- step protocol when 6% DMA was used. This study indicated that from in vitro analysis, TALP with 8% DMSO was the preferred freezing solution for three-yellow cock sperm and this could be carried out using a one-step freezing protocol. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Pandanus amaryllifolius Roxb. Supplementation on Egg Production Performance and Egg Quality Under Different Housing Systems) ผู้เขียน:Suchart Sa-nguanphan สื่อสิ่งพิมพ์:pdf AbstractA 2 ? 2 factorial completely randomized design was conducted to study the effects of Pandanus amaryllifolius Roxb. supplementation under two types of housing system on the egg production performance and egg quality of laying hens. Three hundred-twenty Hisex Brown layers aged about 25 weeks were randomly divided into four groups each with eight replications. The animals in each replicate were randomly fed with a laying-hen diet supplemented with either 0 or 5 g per hen per day of fresh P. amaryllifolius Roxb. and were kept in an evaporative cooling house versus in an open house. The daily egg production was collected over four periods with 28 d per period and the eggs from the last 3 d in each period were collected to measure egg quality. The results showed that the egg production, daily feed intake and egg quality, in terms of egg weight and eggshell thickness, of laying hens kept in the evaporative cooling house were significantly better than those of hens raised under the open house system (P< 0.01). Nevertheless, supplementation with P. amaryllifolius Roxb. in the diet significantly improved the egg production (P< 0.01), whereas the daily feed intake of the hens was also likely to be increased (P = 0.06). The egg production of laying hens kept in the open house system could be improved by about 11% through the supplementation with 5 g per hen per day of P. amaryllifolius Roxb. in the diet, which had no significant effect on the egg production of hens kept in the evaporative cooling house. |